Lehrstuhl für Pflanzenzüchtung

Root Lesion Nematode Resistance in Cereals

Genetic variation for Resistance

 

 

 

 

 

 

 

 

 

 

 

Background

Plant parasitic nematodes from the genus Pratylenchus are major pests in agriculture and attack a wide spectrum of crops including barley, wheat, rape seed, maize, potato, alfalfa, and carrots. The penetration of the nematodes in the roots also leads to secondary infections by fungi or other pathogens that, together with reduction in nutrients- and water-uptake, drastically reduce yield. In the past decade, severe damage in German cereal production has been reported and this pest is regarded by farming experts as a major threat for cereal production. Narrow crop rotation, early-sowing dates, and mild winters which are expected due to climate change increase the damage by this pest.

In a previous work, we have identified several QTL for root lesion nematode resistance in barley (Galal et al. 2014; Sharma et al. 2011, Keil et al. 2009). In this project, we will identify all genes annotated for these intervals and use this information for sequence homology search to different sequence resources of the wheat genome. Based on this analysis, we will identify wheat orthologs of the respective barley candidate genes and test allelic diversity and gene expression in a panel of resistant and susceptible wheat cultivars that will be identified during this project.

Objectives

We are aiming at the identification of QTL for root lesion nematode resistance in wheat. For this, we will establish a PCR based test for measuring RLN infections in cereals that will allow to determine the abundance of RLN in production fields in (northern) Germany and to select wheat genotypes with low infection by RLN adapted to central European climate conditions. At the end, we want to identify the genetic and physiological reasons for RLN resistance in cereals.

Project team

Dr. Gina Capistrano-Goßmann
Jonas Klemm
Brigitte Neidhardt-Olf
Bettina Rohardt
Prof. Dr. Christian Jung

Scientific Partners

Prof. Dr. Johannes Hallmann, Institute for Epidemiology and Pathogen Diagnostics, JKI Münster
http://www.jki.bund.de/no_cache/de/startseite/institute/epidemiologie-pathogendiagnostik/personal/dr-hallmann-johannes.html

Dr. Nils Stein, IPK Gatersleben
http://www.ipk-gatersleben.de/genbank/genomik-genetischer-ressourcen/

Prof. Dr. Frank Ordon, Institute for Resistance Research and Stress Tolerance, JKI Quedlinburg
http://www.jki.bund.de/no_cache/de/startseite/institute/resistenzforschung-stresstoleranz/personal/ordon-frank.html

Prof. Dr. Birgit Classen, Institute of Pharmaceutical Biology, Kiel University
https://www.mnf.uni-kiel.de/de/gleichstellung/gb-mnf/team-1/classen

Other Partners

Dr. Martin Kirchhoff, Dr. Eberhard Laubach, Nordsaat Saatzucht GmbH
http://www.nordsaat.de/

Karin Riemer, Bundessortenamt
http://www.bundessortenamt.de/internet30/index.php?id=3

Publications

Galal A., Sharma S., Abou-Elwafa S. F., Sharma S., Kopisch-Obuch, F., Laubach, E., Perovic D., Ordon F. and Jung C. (2014). Comparative QTL analysis of root lesion nematode resistance in barley. Theor. Appl Genet 127(6): 1399-1407

A. Galal (2014) Mapping Root-Lesion Nematode Resistance QTL in Barley (Hordeum vulgare L.). Doktorarbeit Lehrstuhl Pflanzenzüchtung, Universität Kiel.

Sharma S., S. Sharma, T. Keil, E. Laubach and C. Jung (2011). Screening of barley germplasm for resistance to root lesion nematodes. Plant Genetic Resources 9(02): 236-239.

Sharma S., S. Sharma, F. J. Kopisch-Obuch, T. Keil, E. Laubach, N. Stein, A. Graner and C. Jung (2011). QTL analysis of root-lesion nematode resistance in barley: 1. Pratylenchus neglectus. Theor Appl Genet 122:1321–1330.

Keil T., E. Laubach, S. Sharma and C. Jung (2009). Screening for resistance in the primary and secondary gene pool of barley against the root-lesion nematode Pratylenchus neglectus. Plant Breeding 128; 436-442

T. Keil. Selektion von Gerstenherkünften mit Resistenz gegen freilebende Nematoden der Gattung Pratylenchus. Doktorarbeit Lehrstuhl Pflanzenzüchtung, Universität Kiel. 2008.

Financial Support (start in 2016)

Federal ministry for research and technology (BMBF), Acromyn: NEMARES.

 

Updated: Sep-16               Responsible for this webpage: G. Capistrano-Goßmann