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Molecular Nematode-Plant Interaction
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Objectives:
Plant parasitic nematodes are important pests in many crop species
worldwide. We are aiming at the understanding of plant-nematode interactions
on the molecular-genetic level and at creating novel genetic variation by
introducing resistance genes from different sources.
Results:
The sporamin trypsin inhibitor gene from sweet potato was cloned in sugar
beet by Agrobacterium rhizogenes-mediated transformation. Several root
clones were found to show significant efficiency in inhibiting the growth
and development of the female nematodes.
The inhibition of nematode development was correlated with the trypsin inhibitor activity but not with
the amount of sporamin expressed in the hairy roots.
In a second approach, the cDNA-AFLP technique was used to isolate sugar beet genes involved in the defence response against the beet cyst nematode. A gene with unknown function was found to have a strong inhibitory
effect on female development after cloning into sugar beet roots under the
transcriptional control of the 35S promoter.
Project team:
Daguang Cai
Suren Samuelian
Tina Lange
Tim Thurau
Christian Jung
Partners:
PLANTA GmbH angewandte Pflanzenbiotechnologie, Einbeck
Plant Research International, Wageningen, The Netherlands
Publications:
D. Cai, T. Thurau, Y. Y. Tian, T. Lange, K.-W. Yeh, and C. Jung (2003):
Sporamin-mediated resistance to beet cyst nematodes (Heterodera schachtii
Schm.) is dependent on trypsin inhibitory activity in sugar beet (Beta
vulgaris L.) hairy roots. Plant Mol.Biol., 51: 839-849
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